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Home >
Services
> Custom cDNA Library Construction Services
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Custom cDNA Library Construction Services
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ALS offers high-quality customized cDNA libraries for the specific needs of fellow
researchers. Our staff has over ten years of experience in constructing cDNA and genomic libraries
from a variety of cells and tissues such as:
Human, Mouse, Rat, Monkey, Plant, Algae, Bacteria, Fungi, Yeast, Fruit Fly,
Protozoa, Fish, etc.
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ALS customized cDNA libraries have the following advantages:
- Rigorous mRNA QC steps to test ribosomal RNA and genomic DNA contaminations
- High quality size-fractionated large insert cDNA library (average insert size 1-2 kb)
- Choice of vector for library construction
- Low vector background and high percentage of recombinants (over 90% recombinant)
- Large number of primary clones (3-6 x 106 for standard library and 1-2 x 106 for normalized or subtracted libraries)
- Library transformation into T1 phage resistant E. coli
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Standard cDNA Library Construction
- We guarantee to provide 3-5x106 primary clones
- Average insert size is least 1-1.5 kb and Over 90% recombinants
- The libraries can be made directionally and in the choice of vector for your special needs
- Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
- For quality control purposes we test 10-15 random clones by PCR or restriction digestion
- The library is provided in glycerol or DMSO
Normalized cDNA Libraries
- Normalization is carried out at various Cot levels (Cot10, Cot50 or Cot500) according to relative abundance to genes that needs to be normalized
- Percent Normalization is tested by:
- Relative PCR for house keeping genes and low abundant genes
- Colony/plaque hybridization of 3,000 – 30,000 clones before and after normalization
- DNA sequencing of 96 random cDNA clones
- We guarantee to provide 1x106 primary clones
- Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
- Average inserts size of at least 1 -1.5 kb and over 90% recombinants
- The libraries can be made directionally and in the choice of vector for your specific needs
Subtracted cDNA Libraries
- Subtraction is carried out according to relative abundance to genes that needs to be subtracted
- Percent Subtraction are tested by:
- Relative PCR for house keeping genes and low abundant genes
- Colony/plaque hybridization of 3,000 – 30,000 clones before and after subtraction
- DNA sequencing of 96 random cDNA clones
- We guarantee to provide 1x106 primary clones
- Two size selected cDNA libraries are provided (0.5 - 2 kb and 2+ Kb)
- Average inserts size of at least 1 -1.5 kb and over 90% recombinants
- The libraries can be made directionally and in the choice of vector for your specific needs
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| Ordering Information |
| Item | Catalog Number | Price |
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| Custom Standard cDNA Construction Services | 7110 | $ Please Inquire |
| Custom Normalized cDNA Library Construction Services | 7111 | $ Please Inquire |
| Custom Subtracted cDNA Library Construction Services | 7112 | $ Please Inquire |
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