ALS offers high-quality DNA or isolation services for various applications such as PCR, cloning, cDNA or genomic library construction. Our staff has over ten years of experience in obtaining custom DNA and RNA isolation services from a variety of specimens.

• High quality DNA and RNA for PCR, RT-PCR, Library Construction and Southern/northern Blot Analysis
• DNA and RNA are Free of Proteins and other Contaminants
• Rigorous QC Steps to Ensure the Quality of Nucleic Acid

Genomic DNA Isolation Services

• Cells or tissues are grinded in liquid nitrogen
• Cells or tissues are lysed with Lysozyme, RNase A/T1, Proteinase K and SDS
• DNA is extracted with phenol; phenol: chloroform and dialyzed overnight in TE buffer (3-4 changes)
• A₂₆₀/A₂₈₀ ratio and agarose gel electrophoresis determines the purity of the DNA

RNA and mRNA Isolation Services

• Cells or tissues are grinded in liquid nitrogen
• Total RNA is isolated from cells or tissues using TRIzol®™ Reagents
• mRNA is isolated from total RNA using oligo (dT) – magnetic particles
• A₂₆₀/A₂₈₀ ratio and agarose-formaldehyde gel electrophoresis determines the purity of the RNA and mRNA

We are experienced in isolating DNA and mRNA from the following samples:

• Bacteria
• Algae
• Fungi
• Yeast
• Plants
• Human Tissues
• Mouse/Rat Tissues
• Fish
• Fruit Flies
• Protozoa, etc.