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Buffer for Re-probing Western Blots
American Kife Sciences Western Recycling Buffer System provides ready-to-use reagent for
Stripping Western Blots for Re-probing to be used with another antibody of choice. The buffer doses
not contain any reagent, which has unpleasant smell. The solution is easy to use and removes antibody
within 20 minutes of washing the membrane with only two changes of western recycling buffer. The blots
can be re-probed 3-4 times with 10-30% loss in signal intensities.
Applications and Advantages
- Re-screening Western and Dot Blot of Proteins
- Re-screening Expression cDNA Libraries
- No background
- Ready-to-use Solutions
Kit Components
- 1x Western Recycling Buffer, 150 ml
- 1x Wash Buffer, 1x 100 ml
- Blocking Powder, 1x 10 grams
Storage Condition and Stability
The kit components are stored at RT and stable for six months
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