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Expression & Protein Purification Services

Expression & Protein Purification Services

OUTSOURCE YOUR EXPRESSION AND RECOMBINANT PROTEIN PURIFICATION NEEDS

American Life Sciences offers custom cloning, expression and recombinant protein purification services in E. coli.

Phase I: Sub cloning

  • Sub cloning of your cDNA into bacterial expression vector with His-tag or GST-tag

Phase II: Assessment and Optimization of Expression

  • Expression optimization by transformation into two E. coli host cells and expression optimization at two different temperatures
  • Time course study of expression
  • Small scale protein purification and QC by SDS-PAGE and western blot analysis

Phase III: Recombinant Protein Purification

  • Large Scale growth of best E. coli expressing construct
  • Harvesting cells and cell lysis
  • Protein purification by affinity tag resins
  • Secondary purification on anion exchange resin or by gel filtration (additional charges applies)
  • Dialysis and final QC by SDS-PAGE (>85-90% purified protein)
To discuss custom expression and protein purification project please send inquiries or contact us as follows:

Contact Us

North America: Tel: +1-301-296-8436 Toll Free +1-800-997-2610
Europe: Tel +44-121-227-5283 Toll Free: 0800-015-4624

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